Comparative Evaluation of Specific Activity of Crude and Partially Purified Lipase Preparation from Lipolytic Bacteria

COMPARATIVE EVALUATION OF SPECIFIC ACTIVITY OF CRUDE AND PARTIALLY PURIFIED LIPASE PREPARATION FROM LIPOLYTIC BACTERIA Dr. Kanika Sharma and Madhu Rathore* Address for Correspondence Microbial Research Laboratory, Department of Botany, University College of Science, M.L.S. University Udaipur, Rajasthan – 313001, India E-mail: [email protected] ABSTRACT Most of the medical and commercial applications demands a certain degree of purity in lipase preparations for efficient and successful use hence, lipase from twelve bacterial strains was partially purified by ammonium sulphate precipitation and dialysis method. Agar well diffusion technique as a qualitative method and titrimetric assay as a quantitative method of lipase activity was used. Highest qualitative and quantitative lipolytic activity was observed for crude as well as partially purified lipase from MRL S and MRL 05. Whereas least but significant activity of lipase was observed for strains MRL b and MRL 01.Comparative study of crude and partially purified lipase preparations showed that lipase activity enhanced with concomitant increase in the purity level of lipase. Hence further physicochemical characterisation and purification of lipid degrading principal will be helpful for mass cultivation of these strains as well as for the development of formulations of medical and commercial applications.